There are a number of steps involved in infertility treatment and one step is embryo implant. A multi-cell embryo is filled with fluid blastocyst and implant into the uterus. This implanting process is known as hatching.

Assisted hatching associated with IVF during infertility treatment, many cases have been seen when doctors and couple have to face failure even after attempting ART procedure for a number of times. Assisted hatching is the one test or type of treatment which will help for a couple to have a child. This hatching is a blessing for women who face multiple failure during IVF procedure because of thicker zona shell impairing hatching.

In addition, "assisted hatched embryos implant a day early which allows a greater opportunity for the implant to occur, especially in cases where the endometrium is advanced due to ovarian stimulation."

Types of Assisted Hatching:

In the world, Art specialists have practised four types of assisted hatching, which are described below:

  1. Mechanical assisted hatching: It is also known as Partial Zona dissection. It involves the placing of the embryo at the position by gentle suction. In this procedure, microneedle inserted through ZP at longest private line tangentially. When the cross-shaped slit is created, the embryo will release from its pipette.
  2. Acid Tyrode assisted hatching: In the Zona of the embryo, a defect will be created using mouth controlled acid which is Tyrode on the external surface. There is also need for rinsing the embryos for many times so that excess acid may be washed off and stored when the transfer is complete.
  3. Laser Hatching: In this method of hatching, Zona will pierce by using non-contact mode which is controlled by a computer. Particular software is designed for laser hatching which enables focus and measurement of embryos and finds out an easy position of embryos. For fulfilling all requirements of Zona and its better performance, three laser intensities are used which are low, medium and high. These laser intensities are used to deliver a single pulse within the single click of the mouse.
  4. Assisted hatching by Pronase thinning of Zona pellucid: Diluted solution of Pronase is used in this procedure. For providing initial stretching and softening of ZP, embryos are transferred to Pronase diluted solution in the G2 medium. The main purpose of this method is to make thin ZP without removing it completely.