The purpose of the magnetic cell sorting method or MACS is to enrich a specific cell type depending on their surface antigens (CD molecules) from a mixed population. The adaptability and specificity of magnetic bead cell isolation can be made possible by functionalized bead surfaces which exclusively accept a molecule or antigen (link) on the surface of a target cell. Magnetic beads are framed of a ferrous iron-oxide core surrounded by a polymer shell, or a magnetic ‘pigment’ rooted in a polymer matrix.
The sizes of the beads have an effect on their behavior in a magnetic field. If the beads are small in size, or paramagnetic, meaning that they have no permanent magnetism in zero magnetic fields, but will rapidly become magnetized as well as demagnetized the time application of external magnetic field is made and removed respectively. Magnetic bead cell isolation can be concluded in a single vessel without the requirement for a centrifuge or disposable columns.
More About Magnetic Cell Sorting Method or MACS MACS are the frequently used method of selecting cells by magnetic forces, and was invented and trademarked by Miltenyi Biotec GmbH, Germany. Using this separation technique, the cells of interest are labeled with 50 nanometer diameter, super paramagnetic beads, and sorted with the help of a packed column. Separation can be successfully done by first coating the magnetic beads with an antibody, which is known to selectively hold together to the desired cell type, and incubating them with the sample.
In simple terms, (MACS) magnetic activated cell sorting sperm is a sperm preparation technique that segregates non-apoptotic spermatozoa based on the expression of phosphatidylserine in the membrane of apoptotic sperm. It involves the use of tiny biodegradable magnetic particles attached to an antibody (adnexin V protein). This protein recognizes the sperm that is unhealthy and allows them to be separated from healthy sperm.So, we are able to obtain healthy sperm with the best physiological qualities to be used in assisted reproduction treatments.It is different from conventional semen analysis;
Over the past few years, male infertility has seen a rising curve among people in India and all over the world. The male factor is found in about 40-50% of all infertility cases, and in patients screened at India IVF center, male infertility has seen a jump from 45% in earlier times to nearly 66% in past few years. There are various factors that influence men's being fertile or not. It can be due to variety of reasons like genetic causes, infection, testicular or pelvic injury or a severe medical condition in modern times like diabetes.
- It increases the chances of fertilization of more eggs
- It amounts to formation of better quality of embryos