Dr. Prabhudesai, alumni of TN Medical College-Nair Hospital Mumbai, had his super specialty training in the medical and surgical management of vitreoretinal disease at the prestigious Shankara Nethralaya Chennai. Subsequently, he worked as a vitreoretinal consultant at the same institute, followed by a stint at Shri Ganapati Netralaya Jalna.Since 1994 he has set his own practice in the city of Pune. In 1998.