Diagnostic Tests

Vitamin D3 Test


Vitamin D3 Test

Also known as Vitamin D Profile Blood
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What is this test?

Vitamin D profile test is used to measure the levels of vitamin D in your body.

What is Vitamin-D?

Vitamin D is an essential compound required for proper growth and development of teeth and bones. There are two forms of vitamin D that can be measured in blood. They are 25-hydroxyvitamin D and 1, 25- dihydroxyvitamin D.

The two sources of vitamin D is endogenous or exogenous. Endogenous sources are produced in the skin by the exposure to sunlight. The exogenous sources are through food and supplements. Vitamin D2 and vitamin D3 are the two chemical structures of the Vitamin D. Vitamin D2 is called ergocalciferol which is mostly found in fortified foods and most vitamin preparations and supplements. Vitamin D3 is called cholecalciferol and is produced in the body and also used in some supplements. These two vitamin D2 and vitamin D3 are converted to 1, 25-dihydroxyvitamin D.

Importance of Vitamin-D

The main role of vitamin D is to regulate the levels of phosphorus, calcium, and magnesium in the body. Vitamin D helps in the growth of bones. Vitamin D deficiency makes the bones soft, malformed, and unable to repair and leading to diseases like rickets in children and osteomalacia in adults. It also helps in regulating the immune system and also in the growth of many other tissues. The main sources of vitamin D are fishes like salmon, mackerel, cod liver oil, fortified milk, bread, eggs, and cereals.

Why this test is performed?

This vitamin D test may be recommended to you if you have any abnormal calcium or phosphorus level or abnormal parathyroid levels. This test is also recommended if you have evidence of bone weakness or bone disorders or if you are at high risk of vitamin D deficiency. This test is also performed before starting the treatment for osteoporosis and later to check the progress of treatment. This test is recommended to you if you have a malformation of bones, weakness, osteomalacia, fractures, gastric bypass surgery, malabsorption of fat, people with darker skin, breastfeeding mothers, kidney diseases or sarcoidosis, or lymphomas, etc. This test is also recommended to you if you have a chronic illness such as asthma, psoriasis, or certain immune disorders.

Pathology test parameters:

  • 1, 25-dihydroxyvitamin D (Calcitriol)
  • 25-hydroxyvitamin D (Calcidiol)


Some anti-seizure drugs such as phenytoin may interact with the test results. An overdose of vitamin tablets also interferes with the test results. Hence, inform your doctor or technician about all your current medications and medical conditions prior to the test.

Also known as Vit D3 Test, Vit D Profile Blood, Vitamin D Test, D3 Test, Vitamin D3 Level Test, Vit D Profile, Vitamin D Profile, Vit D3, Vitamin D Total, Vitamin D Total Test.

Test Preparation

Inform your doctor if you are on any medications, have any allergies or underlying medical conditions before your Vitamin D3 Test. Your doctor will give specific instructions depending on your condition on how to prepare for Vitamin D3 Test.

No specific preparation is needed for this test.

Understanding your test results

If your test results show low levels of 25-hydroxyvitamin D, it indicates that you may have vitamin D deficiency. This may be due to low absorption of vitamin D from intestines or low dietary intake of vitamin D in foods or due to less exposure to the sun. If your test results show high levels of 25-hydroxyvitamin D, it may indicate excess dietary intake of vitamin D through nutritional supplements or vitamin tablets.

If your test results show low levels of 1, 25-dihydroxy vitamin D, it may indicate kidney disease or kidney failure. Increase in the levels of 1, 25-dihydroxy vitamin D, may indicate sarcoidosis or lymphomas or excessive parathyroid hormone in the body.

If you receive abnormal test results, consult your health care provider immediately with your test results. Your doctor may recommend further tests, treatment, or lifestyle modifications based on your test results.

GenderAge groupsValue
MALEAll age groups20 - 50 ng/ml
OTHERAll age groups20 - 100ng/ml
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