Diagnostic Tests

Vitamin D 1-25 Hydroxy


Vitamin D 1-25 Hydroxy

Also known as Vitamin D 1-25 Hydroxy Blood
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What is this test?

1, 25-dihydroxy vitamin D test is used to measure the levels of vitamin D in the blood. 1, 25-dihydroxy vitamin D is also called as calcitriol.

What is Vitamin-D?

Vitamin D is an essential compound required for proper growth and development of teeth and bones. There are two forms of vitamin D that can be measured in blood. They are 25-hydroxyvitamin D and 1, 25- dihydroxyvitamin D.

The two sources of vitamin D is endogenous or exogenous. Endogenous sources are produced in the skin by the exposure to sunlight. The exogenous sources are through food and supplements. Vitamin D2 and vitamin D3 are the two chemical structures of the Vitamin D. Vitamin D2 is called ergocalciferol which is mostly found in fortified foods and most vitamin preparations and supplements. Vitamin D3 is called cholecalciferol and is produced in the body and also used in some supplements. These two vitamin D2 and vitamin D3 are converted to 1, 25-dihydroxyvitamin D.

Importance of Vitamin-D

The main role of vitamin D is to regulate the levels of phosphorus, calcium, and magnesium in the body. Vitamin D helps in the growth of bones. Vitamin D deficiency makes the bones soft, malformed, and unable to repair and leading to diseases like rickets in children and osteomalacia in adults. It also helps in regulating the immune system and also in the growth of many other tissues. The main sources of vitamin D are fishes like salmon, mackerel, cod liver oil, fortified milk, bread, eggs, and cereals.

Why this test is performed?

This test may be recommended to you if you have any abnormal calcium or phosphorus level or abnormal parathyroid levels. This test is also recommended if you have evidence of bone weakness or bone disorders or if you are at high risk of vitamin D deficiency. This test is also performed before starting the treatment for osteoporosis and later to check the progress of the treatment. This test is recommended to you if you have a malformation of bones, weakness, osteomalacia, fracture, gastric bypass surgery, malabsorption of fat, people with darker skin, breastfeeding mothers, kidney diseases or sarcoidosis, or lymphomas, etc. This test is also recommended to you if you have a chronic illness such as asthma, psoriasis, or certain immune disorders.


Some anti-seizure drugs such as phenytoin may interact with your test results and an overdose of vitamin tablets also interferes with the test results. Hence, inform your doctor or technician about all your current medications and medical conditions prior to the test.

Test Preparation

Inform your doctor if you are on any medications, have any allergies or underlying medical conditions before your Vitamin D 1-25 Hydroxy. Your doctor will give specific instructions depending on your condition on how to prepare for Vitamin D 1-25 Hydroxy.

No specific preparation is needed for this test.

Understanding your test results

If your test results show low levels of 1, 25-dihydroxyvitamin D, it may indicate kidney disease or kidney failure.

Increase in the levels of 1, 25-dihydroxyvitamin D, may indicate sarcoidosis or lymphomas or excessive parathyroid hormone in the body.

If you receive abnormal test results, consult your health care provider with the test results. Your doctor may recommend further tests, treatments, or lifestyle modifications based on your test results.

GenderAge groupsValue
MALEAll age groupsThe values are reported by the pathologist
FEMALEAll age groupsThe values are reported by the pathologist
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