Sudan Black B (C29H24N6) is a nonfluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections.Sudan Black B is one of the dyes used for Sudan staining. Sudan Black B (SBB) staining of lipids has been studied using model films of polyacrylamide gel containing liposomes prepared from crude lecithine. In these experiments, pure dimethyl sulphoxide was used as solvent for the dye. Sudan Black B dissolved in dimethyl sulphoxide, up to a concentration of nearly 50% (w/v) retaining the molar ratio of the two main components SBB I and SBB II constant over the entire concentration range. The differentiation of model films stained with 2% SBB was carried out in 65% dimethyl sulphoxide. As it proved impossible to find differentiation conditions in which the liposomes remained stained with a completely de-stained background, a mathematical analysis of the differentiation kinetics in the model system was made. This yielded a reliable method for obtaining quantitative date about Sudan Black B staining of liposome-lipids.
No special preparation is needed for Sudan Black B Stain. Inform your doctor if you are on any medications or have any underlying medical conditions or allergies before undergoing Sudan Black B Stain. Your doctor depending on your condition will give specific instructions.
|UNISEX||All age groups||If positive, stains blue black in colour are observed|