Pneumocystis Carinii Stain Toluidine Blue Bronchoalveolar Lavage

A modified toluidine blue O staining technique for Pneumocystis carinii is described. An easily prepared sulfation reagent made with sulfuric and acetic acids was used. The stain can be employed for bronchoalveolar lavages and lung tissue touch preparations. Most background material was removed by the sulfation reagent, slides were generally easy to read, and time from receipt of a specimen to reporting of results was approximately 1 h. P. carinii cysts were more easily visualized with this stain than in slides stained with modified methylene blue, Gram, Gram-Weigert, and standard toluidine blue O procedures. The importance of certain procedural aspects of subsegmental bronchoalveolar lavage, by which most of the specimens were obtained, is also emphasized.

No special preparation is needed for Pneumocystis Carinii Stain Toluidine Blue Bronchoalveolar Lavage. Inform your doctor if you are on any medications or have any underlying medical conditions or allergies before undergoing Pneumocystis Carinii Stain Toluidine Blue Bronchoalveolar Lavage. Your doctor depending on your condition will give specific instructions.