Diagnostic Tests

Hepatitis B Virus Dna Quantitative Pcr Blood


Hepatitis B Virus Dna Quantitative Pcr Blood

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What is this test?

Hepatitis B Virus DNA Quantitative PCR Blood Test measures the amount of Hepatitis B virus DNA (HBV DNA) levels in the blood. This test helps to distinguish active from an inactive infection in Hepatitis B chronic infected individuals. It also helps to monitor and evaluate the treatment response in individuals receiving anti-viral treatment. The HBV DNA fragments are made and measured using Polymerase chain reaction.

What is Hepatitis B Infection?

Hepatitis B infection is an infection of the liver caused by the hepatitis B virus (HBV). This is one type among the other types of viral hepatitis. This infection can spread from one person to another through contact with infected blood and body fluids. Common symptoms of this infection are dark urine, fatigue, fever, abdominal discomfort, weakness, loss of appetite, yellowing of eyes, joint and muscle pain. Symptoms usually appear about one to four months after the occurrence of infection.

Normally in most of the cases, this infection does not last for a long time. Your immune system fights it off within a few months. However, if you have it for more than six months, you are called a carrier. Mostly, adults may recover from this infection fully within a short period of time. Infants and children are more likely to develop a long-lasting infection.

What is Hepatitis B Virus (HBV)?

Hepatitis B Virus (HBV) is a partially double-stranded DNA virus that causes Hepatitis B infection. It belongs to the Hepadnavirus family. HBV contains an outer envelope and an inner core. The outer envelope contains a surface protein called the hepatitis B surface antigen or (HBsAg). The inner core is a protein shell known as the hepatitis B core antigen or (HBcAg). This antigen is consist of hepatitis B virus DNA and enzymes needed for the multiplication of the HBV.

The HBV enters the liver cell of the individuals and is transported into the nucleus of the liver cell. Once it reaches inside the nucleus, the viral DNA is released and replication starts. The virus starts multiplying in the liver cells and this leads to severe infection.

The presence of HBV DNA levels is normally detected in blood by 30 days following infection. It is a reliable marker of active HBV replication (copying).

Why this test is performed?

This test is performed to distinguish active from an inactive HBV infection. This test is also recommended in individuals receiving treatment for Hepatitis B infection to monitor and evaluate the treatment response. This helps to know whether there is any modification is required in the treatment or not. This test helps to differentiate between the active and inactive disease states in chronic HBV infection. This test along with serological tests (HBsAg and HBeAg tests) confirms the presence of chronic Hepatitis B infection.


Contaminated blood samples may give false positive results. In such cases, it is recommended to do a new sample collection and repeat the test.

Test Preparation

Inform your doctor if you are on any medications, have any allergies or underlying medical conditions before your Hepatitis B Virus Dna Quantitative Pcr Blood. Your doctor will give specific instructions depending on your condition on how to prepare for Hepatitis B Virus Dna Quantitative Pcr Blood.

No specific preparation is required for this test.

Understanding your test results

The test results may be vary depending on gender, age, health conditions, and other factors.

If the test results show higher Hepatitis B virus DNA blood levels than the normal range it may indicate that you have active chronic Hepatitis B infection (replicative). If the Hepatitis B virus DNA levels are at moderate levels, it may indicate that you have inactive chronic Hepatitis B infection (nonreplicative). In individuals receiving treatment for hepatitis infection, high levels of Hepatitis B virus DNA indicates a poor response to treatment.

In individuals receiving treatment for hepatitis infection, if the blood sample does not contain Hepatitis B virus DNA or significant low levels it may indicate a more likely or likely response to treatment.

GenderAge groupsValue
UNISEXAll age groups< 10 IU/mL
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