Afb Mtb - Pas Broth Dilution Infected Tissue

Minimum Inhibitory Concentration (MIC) determination is a Quantitative method. Determining MIC using a conventional broth dilution method is a tedious and time consuming process. A defined concentration of antibiotic is loaded on each of the disc so as to form a gradient when placed on agar plate. Broth microdilution is a method used to test the susceptibility of bacteria to antibiotics. During testing, multiple microtiter plates are filled with a broth composed of Brucella and supplements of blood. Varying concentrations of the antibiotics and the bacteria to be tested are then added to the plate. The plate is then placed into a non-CO2 incubator and heated at thirty-five degrees Celsius for sixteen to twenty hours. Following the allotted time, the plate is removed and checked for bacterial growth. If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred. The results of the broth microdilution method are reported in Minimum Inhibitory Concentration (MIC), or the lowest concentration of antibiotics that stopped bacterial expansion. The broth microdilution method can be used to test the susceptibility of bacteria to multiple antibiotics at once.3 Broth microdilution is also highly accurate. The accuracy of its results are comparable to agar dilution, the gold standard of susceptibility testing. Other advantages include the commercial availability of plates, the ease of testing and storing the plates, and the ability for the results of some tests to be read by machines.