Afb Mtb - Kanamycin Disk Diffusion Infected Tissue

Kanamycin (marketed under the brand name Kantrex) is an aminoglycoside antibiotic, available in both oral and intravenous forms, and used to treat a wide variety of infections. Antibiotic complex produced by Streptomyces kanamyceticus from Japanese soil. Comprises 3 components: kanamycin A, the major component, and kanamycins B and C, the minor components. A specific concentration of organism from a culture isolate is applied to an agar plate. Discs of absorbent material to which specific concentrations of antibiotic have been added are placed on the agar. The agar plate is incubated to allow the isolate to grow on the plate in the presence of the antibiotic. If the organism grows to the edge of the impregnated disc, the organism is resistant to that antibiotic. If an area surrounds the disc where growth of the organism has be inhibited, the organism may be susceptible to the antibiotic on the disc. The length of area of inhibition can be measured and compared to standards to determine if the organism is susceptible, intermediate, or resistant to the antibiotic.[Balouiri, Mounyr., Sadiki, Moulay., Ibnsouda, Saad Koraichi.