Acid Fast Bacilli MTb - Kanamycin Antibacterial Gradient Infected Tissue

Kanamycin (marketed under the brand name Kantrex) is an aminoglycoside antibiotic, available in both oral and intravenous forms, and used to treat a wide variety of infections. A gradient strip is a reagent strip that contains a predefined concentration gradient of a specific antibiotic or combination of antibiotics that is used for antimicrobial susceptibility testing (AST) and antimicrobial resistance detection (ARD). For AST, the result is reported in terms of the minimum inhibitory concentration (MIC) of a specific antimicrobial that prevents the visible growth of bacteria. The "Gradient strip" method is used for AST gradient strips that result in an MIC result. A small strip is impregnated with an antimicrobial drug that has an increasing concentration from one end of the strip to another. The concentrations of the antibiotic are marked along the strip. The strip is placed on the agar surface that has been inoculated by a specified concentration of the isolated organism being tested. The minimum inhibitory concentration (MIC) of antibiotics, antifungals, or antimycobacterials is determined by the intersection of the border where microbial growth is inhibited and the concentration of antimicrobial designated on the strip.[CLSI, Methods for Determining Bactericidal Activity of Antimicrobial Agents.