AFB Isolation

A 70% alcohol pad is used to clean the skin A needle is then kept in line with and injected into a vein that can be seen from the skin The blood is dragged out from the needle by a nozzle, saved in a vessel and sealed with your name The sample is then taken to a lab for examination. There are more than 70 species of mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic, granulomatous disease caused by M. tuberculosis. Identification, and, if appropriate, antibiotic sensitivity, is required to initiate necessary therapy. Afb Bactec Culture Blood is done using Bactec Culture method on a Blood sample. This procedure requires the specimen to be collected in blood culture bottles containing culture medium and anticoagulant. Blood cultures are then entered into an automated machine. Rapid and accurate detection of the Mycobacterium tuberculosis complex (MTBC) is a key aspect of effective tuberculosis treatment and control. Although the recently introduced nucleic acid amplification tests provide an opportunity for the early diagnosis of disease, routine culture of acid-fast bacilli (AFB) is still necessary for its higher sensitivity and ability to identify mycobacterial species other than MTBC and for the recovery of isolates for antimicrobial susceptibility testing.