Afb Detection Rrna Tissue Microarray Random Urine

Rapid and accurate detection of Mycobacterium tuberculosis complex (MTBc) is a key aspect of effective tuberculosis treatment and control. The Amplified Mycobacterium tuberculosis complex Direct Test (Gen-Probe) utilizes transcription mediated amplification to detect MTB complex ribosomal RNA. This test is very sensitive, specific, and can be performed within 5 hours. Although it does not differentiate among members of the MTB complex, i.e., M. tuberculosis, M. bovis, M. bovis BCG, M africanum, and M. microti , the isolation of the last 4 organisms is very rare. However, studies have shown that it will also benefit patients with high suspicion of tuberculosis, but with negative AFB smear results. A positive result is strongly suggestive for the diagnosis of tuberculosis if the clinical history and radiographic finding are consistent. This test should not replace AFB smear and culture since they will determine if mycobacterium other than tuberculosis complex are present in addition to MTB complex and the culture is necessary to perform antimycobacterial susceptibility testing. This test has not been studied for use with specimens from patients being treated with antituberculous agents to determine bacteriologic cure or to monitor response to such therapy, and positive results may be caused by the presence of MTBc RNA from nonviable organisms.