Two negative urine tests done at 37 and 45 days after intercourse, with a negative first-morning urine, make pregnancy from that encounter very unlikely. A serum beta-hCG isn't usually needed at this point since urine tests are reliable by six weeks, but your doctor may still check it if there's any doubt.
Next Steps
Since your cycles were previously regular, a missed period like this is often stress-related. Stress can delay ovulation and push a period back by weeks. Your gynaecologist will likely check
thyroid and
prolactin levels and may do a pelvic ultrasound to look for a reason. Book that visit this week rather than waiting longer.
Health Tips
See a gynaecologist sooner if you develop pelvic pain, heavy bleeding or the delay stretches past another month.