Urine
pregnancy test kits are pretty accurate these days but if you want to be 100% sure; you may do a blood test to check for pregnancy
Next Steps
Since it's been two years of trying with evaluation and treatment; it's time for you to take help of assisted reproductive techniques.
Health Tips
To get a better insight into the fertility treatment options you may go thru the following link:http://cheqkmate.com/fertility/solutions/solutions-overview.html
All the best on your quest to parenthood!!