The major crossmatch consists of combining equal volumes (0.1 mL) of the donor RBC suspension and recipient plasma. The control tube contains recipient RBCs and recipient plasma. The samples are incubated, centrifuged, and evaluated for hemolysis or agglutination. Testing of the recipient's (patient) serum with donor cells ("major crossmatch") is required because it is the best available way to detect antibodies in the patient serum that might damage transfused red cells and cause hemolytic transfusion reactions.
Also known as: Major Crossmatch Gel Card Method.