PNH and quantify PNH clones in blood. PNH may be associated with hemolytic anemia, leukopenia, thrombocytopenia and aplastic anemia. This method uses expression of CD55/59 on erythrocytes and the more sensitive fluorescent aerolysin (FLAER) on granulocytes/monocytes. This assay tests for CD59 on erythrocytes using flow cytometry. The percentage of red blood cells (RBCs) with normal expression of CD59 is decreased in paroxysmal nocturnal hemoglobinuria (PNH). The presence of a subclinical PNH population in myelodysplastic bone marrow disorders, such as aplastic anemia or refractory anemia, may correlate with a positive immunotherapeutic response. The lower limit of detection of this test is 0.005 percent PNH cells. This test will distinguish between Type II and Type III cells when the PNH cell percentage is 1 percent or greater. Glycophorin A (CD235a) is used to gate the RBC population. CD59 is the GPI-linked antigen. Recent RBC transfusions may decrease the percentage of PNH cells measured in RBCs.
No special preparation is needed for Cd 55 Immunohistochemistry Blood. Inform your doctor if you are on any medications or have any underlying medical conditions or allergies before undergoing Cd 55 Immunohistochemistry Blood. Your doctor depending on your condition will give specific instructions.
|UNISEX||All age groups||Test is positive if the conditions listed are detected|